Windows Vista’s AutoPlay options are a great improvement over Windows XP in terms of flexibility, but unfortunately there are so many options that it can be confusing, especially since there’s no specific mention of USB Flash drives in the options.
Open your Control Panel, and then click on “Play CDs or other media automatically” to open the AutoPlay dialog.
Disable AutoPlay Globally
The quickest way to disable AutoPlay entirely is to just uncheck the box for “Use AutoPlay for all media and devices”, which should usually work.
Disable for a Single Type
You can choose a setting in the drop-down menu for a single type of drive, for instance Audio CD in this example. For this to work you’ll have to make sure to keep the global autoplay option on, and then choose the specific setting in the drop-down.
Disable for just Removable (flash) Drives
The problem here is that while there are settings for Audio CDs and DVDs, there’s nothing specifically for USB flash drives. Windows will determine the drive type based on the content it finds on the flash drive itself, so that’s what we’ll need to change.
In order to disable AutoPlay for the removable drives, you should change all of the following to Take no action: Software and games, Pictures, Video files, Audio files, and Mixed content.
